Recombinant human 14-3-3 zeta protein
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
32 kDa (282aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gannon-Murakami L., et al. (2002) J Biol Chem. 277 (26) : 23116-23122; ; Li FQ., et al. (2008) J Cell Biol. 181 (7) :1141-54;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Signal Transduction
NCBI Accession Number
NP_663723
Uniprot Accession Number
P63104
Species
Human
AA Sequence
Frequently Asked Questions
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