Recombinant human 14-3-3 theta protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 tau, a subtype of the 14-3-3 family of proteins, was found in T Cells, brain and testes. This 14-3-3 tau is upregulated in patients with amyotrophic lateral sclerosis. Recombinant human YWHAQ was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAQ, 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta, Protein theta

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27 kDa (245aa) confirmed by MALDI-TOF

Additionnal Information

1433 theta, tau, tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein theta polypeptide, YWHAQ, 1C5, HS1, 14-3-3 tau, Tyrosine 3/tryptophan 5-monooxygenase activation protein, theta polypeptide, tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, theta polypeptide 14 3 3 protein T cell, 14 3 3 protein tau, 14 3 3 protein theta, 14 3 3 tau, 14 3 3 theta, KCIP1, Protein HS1, Protein kinase C inhibitor protein 1, tyr3/trp5 monooxygenase activation protein, theta, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, theta polypeptide., YWQ0702-10 µg, YWQ0702-20 µg, YWQ0702-50 µg, YWQ0702-100 µg, YWQ0702-250 µg, YWQ0702-500 µg, YWQ0702-1 mg, YWQ0702-10, YWQ0702-20, YWQ0702-50, YWQ0702-100, YWQ0702-250, YWQ0702-500, YWQ0702-1

References & Citations

Liu YC , et al. (1996) J Biol Chem 271: 14591-5; ; Xiao B , et al. (1995) Nature, 376 188-191;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006817

Uniprot Accession Number

P27348

Species

Human

AA Sequence

MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIDNSQGAYQ EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN