Recombinant human 14-3-3 eta protein
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Product Specifications
Product Name Alternative
YWHAH, YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta, Protein AS1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.3 kDa (266aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sato S., et al. (2006) EMBO J 25 (1) :211-21; ; Chen J., et al. (2007), J Neurosci Res. 85 (8) :1724-33;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_003396
Uniprot Accession Number
Q04917
Species
Human
AA Sequence
Frequently Asked Questions
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