Recombinant human 14-3-3 eta protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAH, YWHA1, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta, Protein AS1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

30.3 kDa (266aa) confirmed by MALDI-TOF

Additionnal Information

1433 eta, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta polypeptide, YWHA1, YWHAH, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide, 14-3-3 eta, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide 14 3 3 protein eta, HGNC:12853, Protein AS1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide, Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide, YWHA 1., YWH0802-10 µg, YWH0802-20 µg, YWH0802-50 µg, YWH0802-100 µg, YWH0802-250 µg, YWH0802-500 µg, YWH0802-1 mg, YWH0802-10, YWH0802-20, YWH0802-50, YWH0802-100, YWH0802-250, YWH0802-500, YWH0802-1

References & Citations

Sato S., et al. (2006) EMBO J 25 (1) :211-21; ; Chen J., et al. (2007), J Neurosci Res. 85 (8) :1724-33;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003396

Uniprot Accession Number

Q04917

Species

Human

AA Sequence

MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACLLAKQA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDE EAGEGN