Recombinant human 14-3-3 gamma protein

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. Recombinant human YWHAG was expressed in E.coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAG, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein gamma N-terminally processed, protein phosphatase 1, regulatory subunit 170, PPP1R170, 14-3-3γ

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

28 kDa (247aa) confirmed by MALDI-TOF

Additionnal Information

1433 g, 1433 gamma, 1-4-3-3, gamma, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein gamma polypeptide, YWHAG, 14-3-3 gamma, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, gamma polypeptide 14 3 3 protein gamma, 14 3 3 protein gamma subtype, 14 3 3gamma, 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide, KCIP 1, KCIP1, Protein kinase C inhibitor protein 1, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide., YWG0701-10 µg, YWG0701-20 µg, YWG0701-50 µg, YWG0701-100 µg, YWG0701-250 µg, YWG0701-500 µg, YWG0701-1 mg, YWG0701-10, YWG0701-20, YWG0701-50, YWG0701-100, YWG0701-250, YWG0701-500, YWG0701-1

References & Citations

Chen XQ, et al. (2005) Glia. 42 (4) :315-24 ; ; Chen XQ, Yu AC, et al. (2002) Biochem Biophys Res Commun. 296 (3) :657-63 ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036611

Uniprot Accession Number

P61981

Species

Human

AA Sequence

MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN