Recombinant human 14-3-3 beta/alpha protein

The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human 14-3-3 beta was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

YWHAB, YWHAA, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha N-terminally processed, GW128, HEL-S-1, HS1, KCIP-1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28 kDa (246aa) confirmed by MALDI-TOF

Additionnal Information

1433 b, 1433 beta, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein beta polypeptide, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide, 14-3-3 beta, YWHAB, GW128, HS1, KCIP-1, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide 14 3 3 protein beta/alpha, Brain protein 14 3 3 beta isoform, GW 128, HS 1, KCIP 1, KCIP1, Protein 1054, Protein kinase C inhibitor protein 1, Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide., YWB0801-10 µg, YWB0801-20 µg, YWB0801-50 µg, YWB0801-100 µg, YWB0801-250 µg, YWB0801-500 µg, YWB0801-1 mg, YWB0801-10, YWB0801-20, YWB0801-50, YWB0801-100, YWB0801-250, YWB0801-500, YWB0801-1

References & Citations

Rodriguez LG., et al. (2005) J Cell Physiol. 202 (1) :285-94.; ; Mils V., et al. (2000) Oncogene. 19 (10) :1257-65.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 50 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003395.1

Uniprot Accession Number

P31946

Species

Human

AA Sequence

MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN