Recombinant mouse visfatin/NAMPT protein

Visfatin is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (pre-B cell colony-enhancing factor, PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Recombinant mouse visfatin was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pre-B-cell colony-enhancing factor 1, Pre-B-cell colony-enhancing factor 1, Visfatin, visfatin mouse, mouse visfatin, Nicotinamide phosphoribosyltransferase [Mus musculus], Pre-B-cell colony-enhancing factor 1, Uscropma-cell colony-enhancing 2

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

57.6 kDa (511aa) confirmed by MALDI-TOF

Additionnal Information

PBEF, Visfatin, Pre-B-cell colony-enhancing factor 1, Pre-B-cell colony-enhancing factor 1, Visfatin, visfatin mouse, mouse visfatin, Nicotinamide phosphoribosyltransferase [Mus musculus], Pre-B-cell colony-enhancing factor 1, VIS0702-10 µg, VIS0702-20 µg, VIS0702-50 µg, VIS0702-100 µg, VIS0702-250 µg, VIS0702-500 µg, VIS0702-1 mg, VIS0702-10, VIS0702-20, VIS0702-50, VIS0702-100, VIS0702-250, VIS0702-500, VIS0702-1

References & Citations

Stephens JM., et al. (2006) Curr Opin Lipidol. 17:128-31.; ; Hug C., et al. (2005) Science. 307 (5708) :366-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_067499.2

Uniprot Accession Number

Q99KQ4

Species

Mouse

AA Sequence

MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H