Recombinant human VHL protein

Von Hippel-Lindau disease (VHL) is a dominant inherited syndrome characterized by the predisposition to develop various kinds of benign and malignant tumors, including clear cell renal carcinomas, pheochromocytomas and hemangioblastomas of the central nervous system and retina. VHL syndrome is caused by germline mutation in the VHL tumor suppressor, and VHL tumors are associated with loss or mutation of the remaining wild-type allele. VHL has two domains: a roughly 100-residue NH2-terminal domain rich in beta sheet (beta-domain) and a smaller alpha-helical domain (alpha-domain), held together by two linkers and a polar interface. VHL protein is also involved in the degradation of hypoxia-inducible factor (HIF) . VHL beta-domain (1-154aa) was overexpressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1, HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin binding protein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, von Hippel Lindau syndrome, von Hippel Lindau tumor suppressor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

19.2 kDa (174aa) confirmed by MALDI-TOF

Additionnal Information

VHL, Von Hippel-Lindau tumor suppressor isoform 1, Von Hippel-Lindau tumor suppressor isoform 1, HRCA1, RCA1, VHL1, Von Hippel-Lindau tumor suppressor isoform 1 pVHL, G7 protein, Elongin binding protein, HRCA 1, RCA 1, VHL, VHL 1, VHLH, Von Hippel Lindau disease tumor suppressor, von Hippel Lindau syndrome, von Hippel Lindau tumor suppressor, VHL3001-10 µg, VHL3001-20 µg, VHL3001-50 µg, VHL3001-100 µg, VHL3001-250 µg, VHL3001-500 µg, VHL3001-1 mg, VHL3001-10, VHL3001-20, VHL3001-50, VHL3001-100, VHL3001-250, VHL3001-500, VHL3001-1

References & Citations

Latif F., et al. (1993) Science. 260 (5112) :1317-20; ; Duan DR., et al. (1995) PNAS. 92 (14) :6459-63; ; Maxwell PH., et al. (1999) Nature 399 (6733) :271-5;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. phosphate-buffered Saline (pH 7.4) containing 1mM DTT, 2mM EDTA

Scientific Category

Cancer

NCBI Accession Number

NP_000542.1

Uniprot Accession Number

P40337

Species

Human

AA Sequence

MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFR DAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLP