Recombinant human Serpin A12/Vaspin protein

Vaspin (visceral adipose-specific SERPIN), a newly identified adipokine, which is a member of serine protease inhibitor family. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that induction of human vaspin mRNA expression in adipose tissue is regulated in a fat depot-specific manner and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. Recombinant human Vaspin fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, SERPINA12, serpin peptidase inhibitor clade A member 12, OL-64, Visceral adipose tissue-derived serine protease inhibitor, Serpin A12 precursor, OL64 OL 64, ENSG00000165953, Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 antibody

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.4 kDa (415aa) confirmed by MALDI-TOF

Additionnal Information

Serpin A12, Vaspin, Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, SERPINA12, serpin peptidase inhibitor clade A member 12, OL-64, Visceral adipose tissue-derived serine protease inhibitor, Serpin A12 precursor, OL64 OL 64, ENSG00000165953, Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 antibody, VAS0701-10 µg, VAS0701-20 µg, VAS0701-50 µg, VAS0701-100 µg, VAS0701-250 µg, VAS0701-500 µg, VAS0701-1 mg, VAS0701-10, VAS0701-20, VAS0701-50, VAS0701-100, VAS0701-250, VAS0701-500, VAS0701-1

References & Citations

Hida K., et al. (2005) PNAS. 102 (30) :10610-5.; ; Kloting N., et al. (2006) Biochem Biophys Res Commun. 339 (1) :430-6;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2mM PMSF, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_776249.1

Uniprot Accession Number

Q8IW75

Species

Human

AA Sequence

LKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK