Recombinant human Cellubrevin/VAMP-3 protein
Cellubrevin, also known as VAMP 3, is present in recycling endosomes and endosome-derived vesicles. This protein has been implicated in recycling of transferrin receptors to the plasma membrane, secretion of alpha-granules in platelets, recycling of T-cell receptors to the immunological synapses, and membrane trafficking during cell migration. Recombinant cellubrevin was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Vesicle-associated membrane protein 3, VAMP 3, CEB, Synaptobrevin-3, Cellubrevin, Synaptobrevin 3, VAMP3, Vesicle associated membrane protein 3.
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
8.7 kDa (77aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hu C., et al. (2007) Exp Cell Res. 313 (15) : 3198-209.; ; Zhang H., et al. (2007) Arch Biochem biophys. 465 (1) :119-26.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_004772
Uniprot Accession Number
Q15836
Species
Human
AA Sequence
MSTGPTAATG SNRRLQQTQN QVDEVVDIMR VNVDKVLERD QKLSELDDRA DALQAGASQF ETSAAKLKRK YWWKNCK
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