Recombinant human VAMP-1 protein

Synaptobrevin 1 (Vehicle-associated membrane, VAMP1) is one of the key proteins in the SNARE complex which is involved in regulated exocytosis. Synaptobrevin1 binds to t-SNAREs, syntaxin (STX) and SNAP25, after the fusion of synaptic vesicles to plasma membrane. Recombinant human Synaptobrevin1, fused to His- tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Vesicle-associated membrane protein 1 isoform 1, VAMP1, SYB1, Synaptobrevin 1, VAMP-1, Synaptobrevin-1, Vesicle-associated membrane protein 1, Vesicle-associated membrane protein 1 isoform 1 DKFZp686H12131, SYB 1, Synaptobrevin1, VAMP 1, Vesicle associated membrane protein 1, Vesicle associated membrane protein 1 synaptobrevin 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

11.9 kDa (111aa) confirmed by MALDI-TOF

Additionnal Information

VAMP1, Vesicle-associated membrane protein 1 isoform 1, VAMP1, SYB1, Synaptobrevin 1, VAMP-1, Synaptobrevin-1, Vesicle-associated membrane protein 1, Vesicle-associated membrane protein 1 isoform 1 DKFZp686H12131, SYB 1, Synaptobrevin1, VAMP 1, Vesicle associated membrane protein 1, Vesicle associated membrane protein 1 synaptobrevin 1., VAM0711-10 µg, VAM0711-20 µg, VAM0711-50 µg, VAM0711-100 µg, VAM0711-250 µg, VAM0711-500 µg, VAM0711-1 mg, VAM0711-10, VAM0711-20, VAM0711-50, VAM0711-100, VAM0711-250, VAM0711-500, VAM0711-1

References & Citations

Nystuen AM., et al. (2007) Neurogenetics. 8:1-10.; ; Pennuto M., et al. (2003) Mol Biol Cell. 14:4909-19.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM EDTA

Scientific Category

Neuroscience

NCBI Accession Number

NP_055046

Uniprot Accession Number

P23763

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY W

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