Recombinant human VAMP-1 protein
Synaptobrevin 1 (Vehicle-associated membrane, VAMP1) is one of the key proteins in the SNARE complex which is involved in regulated exocytosis. Synaptobrevin1 binds to t-SNAREs, syntaxin (STX) and SNAP25, after the fusion of synaptic vesicles to plasma membrane. Recombinant human Synaptobrevin1, fused to His- tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
11.9 kDa (111aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nystuen AM., et al. (2007) Neurogenetics. 8:1-10.; ; Pennuto M., et al. (2003) Mol Biol Cell. 14:4909-19.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM EDTA
Scientific Category
Neuroscience
NCBI Accession Number
NP_055046
Uniprot Accession Number
P23763
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSAPAQPPAE GTEGTAPGGG PPGPPPNMTS NRRLQQTQAQ VEEVVDIIRV NVDKVLERDQ KLSELDDRAD ALQAGASQFE SSAAKLKRKY W
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