Recombinant human UbcH8/UBE2L6 protein
Ubiquitin-conjugating enzyme E2L6 (uBE2L6), also known as ubcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by uBE2L3 gene. Recombinant human uBE2L6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
21.7 kDa (188aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ardley HC., et al. (2000) Cytogenet Cell Genet. 89 (1-2) :137-140.; ; Movnihan TP., et al. (1999) J Biol Chem. 274 (43) :30963-30968. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1mM PMSF, 10% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_004214
Uniprot Accession Number
O14933
Species
Human
AA Sequence
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