Recombinant human UbcH7/UBE2L3 protein

Human ubquitin-conjugating enzyme 7 (ubcH7) is a ubiquitin-conjugating enzyme (E2) mediating c-fos degradation, transcription factor NF-kappa B maturation, and human papilloma virus-mediated p53 and Myc protein degradation, in vitro. The ubiquitin-conjugating enzymes (E2s) are essential components of the post-translational protein ubiquitination pathway, mediating the transfer of activated ubiquitin to substrate proteins. The human uBE2L1-uBE2L4 gene could potentially encode different isoforms of the ubcH7. uBE2L3 gene, located at chromosome 22q11. 2, is the only identical family member with introns and encodes a polypeptide sequence identical to that of ubcH7. ubcH7 (154amino acid) was over-expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2 L3, E2 ubiquitin-conjugating enzyme L3, L-UBC, UbcH7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-protein ligase L3, UBCE7, UBCH7

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.9 kDa (154aa) confirmed by MALDI-TOF

Additionnal Information

Ubiquitin-protein ligase L3, ubiquitin-protein ligase G1, ubiquitin-conjugating enzyme E2L 3 uBC 7, ubiquitin-conjugating enzyme E2L 3, Ubiquitin-conjugating enzyme E2-F1, Ubiquitin-conjugating enzyme E2 L3, ubiquitin-conjugating enzyme E2 G1, ubiquitin protein ligase G1, ubiquitin conjugating enzyme E2L 3 uBC 7, ubiquitin conjugating enzyme E2L 3, ubiquitin conjugating enzyme E2G 1 (uBC7 homolog yeast), ubiquitin conjugating enzyme E2G 1 (uBC7 homolog C. elegans) antibody, ubiquitin conjugating enzyme E2G 1 (homologous to C. elegans uBC7), ubiquitin conjugating enzyme E2G 1, Ubiquitin conjugating enzyme E2 L3, ubiquitin conjugating enzyme E2 G1, Ubiquitin conjugating enzyme E2 F1, Ubiquitin carrier protein L3, ubiquitin carrier protein G1, uBE2G1, uBE2G, uBE 2G, UBCH7, UBCE7, uBC7 homolog yeast, ubc7 (ubiquitin Conjugating enzyme 7), uBC7, L-UBC, EC 6.3.2.19, E217K, E2 ubiquitin-conjugating enzyme L3, E2 ubiquitin conjugating enzyme L3, UBC3003-10 µg, UBC3003-20 µg, UBC3003-50 µg, UBC300

References & Citations

Ardley HC., et al. (2000) Biochim Biophys Acta. 1491 (1-3) :57-64.; ; Moynihan TP., et al. (1998) Genomics. 51 (1) :124-127.; ; Nuber u., et al (1996) J Biol Chem. 271 (5) :2795-2800;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM HEPES buffer (pH 7.4) containing 150 mM NaCl, 1mM DTT, 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_003338

Uniprot Accession Number

P68036

Species

Human

AA Sequence

MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD