Recombinant human Ubc9/UBE2I protein

Human ubc9 is homologous to ubiquitin-conjugating enzymes (E2s) . However, instead of conjugating ubiquitin, it conjugates a ubiquitin homologue, small ubiquitin-like modifier 1 (SuMO-1) . And hubc9 retains striking structural and functional conservation with yeast ubc9. The ubiquitin-dependent protein degradation system has been recognized as a complete enzymatic pathway that is responsible for the selective degradation of abnormal and short-lived proteins. The conjugation of ubiquitin reuires the activities of ubiquitin-activating (E1) and -conjugating (E2) enzymes.

Product Specifications

Product Name Alternative

Ubiquitin conjugating enzyme E2 I, SUMO-conjugating enzyme UBC9, ING-type E3 SUMO transferase UBC9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBCE9

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

18 kDa (158aa) confirmed by MALDI-TOF

Additionnal Information

UBE2I, Ubc9, UBC3001-10 µg, UBC3001-20 µg, UBC3001-50 µg, UBC3001-100 µg, UBC3001-250 µg, UBC3001-500 µg, UBC3001-1 mg, UBC3001-10, UBC3001-20, UBC3001-50, UBC3001-100, UBC3001-250, UBC3001-500, UBC3001-1

References & Citations

Tatham MH., et al (2003) Biochemistry. 42 (11) 3168-3179; ; Donghai Lin, et al (2002) J. Biol. Chem. 277 (24) 21740-21748.; ; Wang ZY., et al. (1996) J. Biol. Chem. 271 (40) :24811-6.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM HEPES buffer (pH 7.4) containing 150 mM NaCl, 1mM DTT, 10%glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_003336

Uniprot Accession Number

P63279

Species

Human

AA Sequence

MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA QAKKFAPS