Recombinant human Thioredoxin-2 protein

Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Recombinant Thioredoxin2 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >40 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Product Name Alternative

MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >40 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Molecular Weight

11 kDa (108aa) confirmed by MALDI-TOF

Additionnal Information

Thioredoxin2, MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2, TRX0801-10 µg, TRX0801-20 µg, TRX0801-50 µg, TRX0801-100 µg, TRX0801-250 µg, TRX0801-500 µg, TRX0801-1 mg, TRX0801-10, TRX0801-20, TRX0801-50, TRX0801-100, TRX0801-250, TRX0801-500, TRX0801-1

References & Citations

Wang Z., et al. (2007) . Rapid Commun Mass Spectrom. 21 (22) :3658-66.; ; Zhang H., et al. (2007) . Arch Biochem biophys. 465 (1) :119-26.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Redox Proteins

NCBI Accession Number

NP_036605

Uniprot Accession Number

Q99757

Species

Human

AA Sequence

MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG

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