Recombinant human Thioredoxin-1 protein

Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Specifications

Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Product Name Alternative

TRX1, TRX2, Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, Thioredoxin-1, ADF, Surface associated sulphydryl protein, TXN protein, ATL derived factor, DKFZp686B1993, MGC61975, SASP, Thioredoxin, TRDX, TRX, TRX 1, TXN

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Molecular Weight

11.7 kDa (105aa) confirmed by MALDI-TOF

Additionnal Information

Thioredoxin1, TRX1, TRX2, Thioredoxin-1, Thioredoxin I, TR-I, Thioredoxin-2, Thioredoxin-1, ADF, Surface associated sulphydryl protein, TXN protein, ATL derived factor, DKFZp686B1993, MGC61975, SASP, Thioredoxin, TRDX, TRX, TRX 1, TXN, TRX0501-10 µg, TRX0501-20 µg, TRX0501-50 µg, TRX0501-100 µg, TRX0501-250 µg, TRX0501-500 µg, TRX0501-1 mg, TRX0501-10, TRX0501-20, TRX0501-50, TRX0501-100, TRX0501-250, TRX0501-500, TRX0501-1

References & Citations

Pigiet VP., et al. (1986) Proc. Natl.Acad.Sci. uSA 83 (20) :7643-7.; ; Lundstrom J., et al. (1990) J.Biol.Chem. 265 (16) :9114-20.; ; Laurent TC., et al. (1964) J Biol Chem. 239:3436-44.;