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Recombinant human TNF-alpha protein

Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.

Product Specifications

Specifications

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/mL.

Molecular Weight

17.5 kDa (158aa) confirmed by MALDI-TOF

Additionnal Information

TNF-a, Tumor necrosis factor alpha, TNFA, TNFSF2, DIF (differentiation inducing factor), CACHECTIN, DIF, TNF-alpha, Tumor necrosis factor alpha, Tumor necrosis factor alpha, TNF, tumor necrosis factor (TNF superfamily, member 2), TNF superfamily member 2, TNF0501-10 µg, TNF0501-20 µg, TNF0501-50 µg, TNF0501-100 µg, TNF0501-250 µg, TNF0501-500 µg, TNF0501-1 mg, TNF0501-10, TNF0501-20, TNF0501-50, TNF0501-100, TNF0501-250, TNF0501-500, TNF0501-1

References & Citations

Chicoine M.R., et al. (1997), J. Neurooncol. 35: 249-57; ; Ruddle N.H., et al. (1992), Curr. Opin. Immunol. 4: 327-32; ; Wingfield P., et al. (1987), FEBS Lett. 211:179-184;

Other References

Kim JA et al., A new lupane-type triterpene from the seeds of Panax ginseng with its inhibition of NF-κB. (Arch Pharm Res. 2012) {https://pubmed.ncbi.nlm.nih.gov/22553057/}; ; Nhiem NX et al., Inhibition of nuclear transcription factor-κB and activation of peroxisome proliferator-activated receptors in HepG2 cells by cucurbitane-type triterpene glycosides from Momordica charantia. (J Med Food. 2012) {https://pubmed.ncbi.nlm.nih.gov/22248180/}; ; Song SB et al., Inhibition of TNF-α-mediated NF-κB Transcriptional Activity in HepG2 Cells by Dammarane-type Saponins from Panax ginseng Leaves. (J Ginseng Res. 2012) {https://pubmed.ncbi.nlm.nih.gov/23717114/}; ; Liang C et al., Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB. (J Ginseng Res. 2013) {https://pubmed.ncbi.nlm.nih.gov/23717159/}; ; Cho K et al., Inhibition of TNF-α-Mediated NF-κB Transcriptional Activity by Dammarane-Type Ginsenosides from Steamed Flower Buds of Panax ginseng in HepG2 and SK-Hep1 Cells. (Biomol Ther (Seoul) . 2014) {https://pubmed.ncbi.nlm.nih.gov/24596622/}; ; Thao NP et al., Inhibition of NF-κB transcriptional activation in HepG2 cells by diterpenoids from the soft coral Sinularia maxima. (Arch Pharm Res. 2014) {https://pubmed.ncbi.nlm.nih.gov/23934574/}; ; Thao NP et al., NF-κB inhibitory activity of polyoxygenated steroids from the Vietnamese soft coral Sarcophyton pauciplicatum. (Bioorg Med Chem Lett. 2014) {https://pubmed.ncbi.nlm.nih.gov/24852121/}; ; Li W et al., Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana. (Biomol Ther (Seoul) . 2014) {https://pubmed.ncbi.nlm.nih.gov/25143813/}; ; He F et al., Antitumor effects of dammarane-type saponins from steamed Notoginseng. (Pharmacogn Mag. 2014) {https://pubmed.ncbi.nlm.nih.gov/25210319/}; ; Yang SY et al., NF-κB Activation and PPAR Transactivational Effects of a New Aliphatic Acid Amide from Pericarps of Zanthoxylum piperitum. (Bull Korean Chem Soc. 2014) {https://doi.org/10.5012/bkcs.2014.35.8.2361}; ; Sun YN et al., NF-κB Inhibitory Activities of Phenolic and Lignan Components from the Stems of Acanthopanax divaricatus var. albeofructus. (Natural Product Sciences. 2014) {https://koreascience.kr/article/JAKO201402852974800.page}; ; Sun YN et al., Nuclear Factor Kappa B Activation and Peroxisome Proliferator-activated Receptor Transactivational Effects of Chemical Components of the Roots of Polygonum multiflorum. (Pharmacogn Mag. 2016) {https://pubmed.ncbi.nlm.nih.gov/27019559/}; ; Sun YN et al., Isolation and identification of chromone and pyrone constituents from Aloe and their anti-inflammatory activities. (J Funct Foods. 2016) {https://doi.org/10.1016/j.jff.2015.12.026}; ; Sun YN et al., A new phenolic derivative with soluble epoxide hydrolase and nuclear factor-kappaB inhibitory activity from the aqueous extract of Acacia catechu. (Nat Prod Res. 2016) {https://pubmed.ncbi.nlm.nih.gov/26647286/}; ; Kwak MS et al., Peroxiredoxin-mediated disulfide bond formation is required for nucleocytoplasmic translocation and secretion of HMGB1 in response to inflammatory stimuli. (Redox Biol. 2019) {https://pubmed.ncbi.nlm.nih.gov/31026770/}; ; Jang SH et al., Sinapic acid alleviates inflammatory bowel disease (IBD) through localization of tight junction proteins by direct binding to TAK1 and improves intestinal microbiota. (Front Pharmacol. 2023) {https://pubmed.ncbi.nlm.nih.gov/37649894/}; ; Kim S et al., Hybrid nutraceutical of 2-ketoglutaric acid in improving inflammatory bowel disease: Role of prebiotics and TAK1 inhibitor. (Biomed Pharmacother. 2024) {https://pubmed.ncbi.nlm.nih.gov/38219386/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000585.2

Uniprot Accession Number

P01375

Species

Human

AA Sequence

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
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