Recombinant human TNF-alpha protein
Tumor necrosis factor alpha (TNF-alpha), also called cachectin, consists of 157 amino acids. TNF-alpha is a 17. 5 kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein.
Product Specifications
Specifications
Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
Product Name Alternative
Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
17.5 kDa (158aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000585.2
Uniprot Accession Number
P01375
Species
Human
AA Sequence
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