Recombinant human Syntaxin 1A protein
Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE (H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex. Recombinant syntaxin 1A protein (1-226aa) contains N-terminal domain (Habc) and t_SNARE domain (H3 domain) and this protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.
Product Specifications
Product Name Alternative
STX1A, STX1, Syntaxin 1A, Neuron-specific antigen HPC-1, Syntaxin-1A, HPC 1, Neuron specific antigen HPC1, STX1, Syntaxin 1A brain.
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.1 kDa (226aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Han X., et al. (2004) Science. 304:289-292; ; Woodbury DJ. et al. (2000) Cell Biology International. 24 (11) :809-818.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_004594.1
Uniprot Accession Number
Q16623
Species
Human
AA Sequence
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