Recombinant human Syntaxin 1A protein

Syntaxin is membrane integrated Q-SNARE protein participating in exocytosis. Syntaxin is composed of an N-terminal regulatory domain (Habc), a SNARE domain (known as H3), and a single C-terminal transmembrane domain. The SNARE (H3) domain binds to both synaptobrevin and SNAP-25 forming the core SNARE complex. Recombinant syntaxin 1A protein (1-226aa) contains N-terminal domain (Habc) and t_SNARE domain (H3 domain) and this protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.

Product Specifications

Product Name Alternative

STX1A, STX1, Syntaxin 1A, Neuron-specific antigen HPC-1, Syntaxin-1A, HPC 1, Neuron specific antigen HPC1, STX1, Syntaxin 1A brain.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.1 kDa (226aa) confirmed by MALDI-TOF

Additionnal Information

Syntaxin1A, STX1A, STX1, Syntaxin 1A, Neuron-specific antigen HPC-1, Syntaxin-1A, HPC 1, Neuron specific antigen HPC1, STX1, Syntaxin 1A brain., STX0706-10 µg, STX0706-20 µg, STX0706-50 µg, STX0706-100 µg, STX0706-250 µg, STX0706-500 µg, STX0706-1 mg, STX0706-10, STX0706-20, STX0706-50, STX0706-100, STX0706-250, STX0706-500, STX0706-1

References & Citations

Han X., et al. (2004) Science. 304:289-292; ; Woodbury DJ. et al. (2000) Cell Biology International. 24 (11) :809-818.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_004594.1

Uniprot Accession Number

Q16623

Species

Human

AA Sequence

MKDRTQELRT AKDSDDDDDV AVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQ