Recombinant streptomyces avidinii Streptavidin protein

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.

Expression System

E.coli

Antigen Species

Streptomyces, Streptomyces avidinii

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.5 kDa (167aa) confirmed by MALDI-TOF

Additionnal Information

Streptavidin, Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2., STV0801-10 µg, STV0801-20 µg, STV0801-50 µg, STV0801-100 µg, STV0801-250 µg, STV0801-500 µg, STV0801-1 mg, STV0801-10, STV0801-20, STV0801-50, STV0801-100, STV0801-250, STV0801-500, STV0801-1

References & Citations

Howarth M., et al. (2006) Nat Methods. 3 (4) :267-73.; ; Holmberg A., et al (2005) . Electrophoresis. 26 (3) :501-10.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

CAA27265.1

Uniprot Accession Number

P22629

Species

Streptomyces, Streptomyces avidinii

AA Sequence

DP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ