Recombinant streptomyces avidinii Streptavidin protein
Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.
Expression System
E.coli
Antigen Species
Streptomyces, Streptomyces avidinii
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
17.5 kDa (167aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Howarth M., et al. (2006) Nat Methods. 3 (4) :267-73.; ; Holmberg A., et al (2005) . Electrophoresis. 26 (3) :501-10.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5)
Scientific Category
Fusion Tag & Detection
NCBI Accession Number
CAA27265.1
Uniprot Accession Number
P22629
Species
Streptomyces, Streptomyces avidinii
AA Sequence
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