Recombinant human STIM1 protein

Stromal interaction molecule 1 (STIM1) is a transmembrane protein that is essential for store-operated Ca (2+) entry, a process of extracellular Ca (2+) influx in response to the depletion of Ca (2+) stores in the endoplasmic reticulum (ER) . STIM1 localizes predominantly to the ER; upon Ca (2+) release from the ER, STIM1 translocates to the ER-plasma membrane junctions and activates Ca (2+) channels. Recombinant Calmodulin tagged STIM was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Stromal interaction molecule 1, GOK, D11S4896E, Stromal interaction molecule 1, STIM1, Stromal interaction molecule 1 D11S4896E, SIM, STIM 1, Stim1 stromal interaction molecule 1.

Expression System

E.coli

Antigen Species

Human

Tag

Calmodulin Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38 kDa (343aa) confirmed by MALDI-TOF

Additionnal Information

STIM1, Stromal interaction molecule 1, GOK, D11S4896E, Stromal interaction molecule 1, STIM1, Stromal interaction molecule 1 D11S4896E, SIM, STIM 1, Stim1 stromal interaction molecule 1., STI0801-10 µg, STI0801-20 µg, STI0801-50 µg, STI0801-100 µg, STI0801-250 µg, STI0801-500 µg, STI0801-1 mg, STI0801-10, STI0801-20, STI0801-50, STI0801-100, STI0801-250, STI0801-500, STI0801-1

References & Citations

Varnai P., et al. (2007) J. Biol. Chem . 282 (40) :29678-90.; ; Grigoriev I., et al. (2008) Curr Biol. 18 (3) :177-82.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003147

Uniprot Accession Number

Q13586

Species

Human

AA Sequence

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SMLSHSHSEK ATGTSSGANS EESTAAEFCR IDKPLCHSED EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL WKAWKSSEVY NWTVDEVVQW LITYVELPQY EETFRKLQLS GHAMPRLAVT NTTMTGTVLK MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKD