Recombinant human SOD1/Cu-Zn SOD protein

Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS) . Recombinant SOD1 was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 500 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Product Name Alternative

Superoxide dismutase 1 soluble, ALS, SOD, ALS1, IPOA, Cu-Zn superoxide dismutase, Superoxide dismutase 1 soluble, SOD1, Superoxide dismutase 1, soluble ALS 1, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult, Cu/Zn SOD, Cu/Zn superoxide dismutase, Homodimer, Indophenoloxidase A, Mn superoxide dismutase, SOD 1, SOD soluble, SOD2, SODC, Superoxide dismutase 1 soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 500unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Molecular Weight

15.9 kDa (154aa) confirmed by MALDI-TOF

Additionnal Information

SOD1, Cu-Zn SOD, Superoxide dismutase 1 soluble, ALS, SOD, ALS1, IPOA, Cu-Zn superoxide dismutase, Superoxide dismutase 1 soluble, SOD1, Superoxide dismutase 1, soluble ALS 1, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult, Cu/Zn SOD, Cu/Zn superoxide dismutase, Homodimer, Indophenoloxidase A, Mn superoxide dismutase, SOD 1, SOD soluble, SOD2, SODC, Superoxide dismutase 1 soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic, SOD0801-10 µg, SOD0801-20 µg, SOD0801-50 µg, SOD0801-100 µg, SOD0801-250 µg, SOD0801-500 µg, SOD0801-1 mg, SOD0801-10, SOD0801-20, SOD0801-50, SOD0801-100, SOD0801-250, SOD0801-500, SOD0801-1

References & Citations

Conwit R., et al. (2006) Journal of the Neurological Sciences251 (1-2) .; ; Banci L., et al. (2008) PLoS ONE. 3 (2) :e1677.

Other References

Kwon MJ, et al. Superoxide Dismutase 3 Controls Adaptive Immune Responses and Contributes to the Inhibition of Ovalbumin-Induced Allergic Airway Inflammation in Mice. (Antioxid Redox Signal. 2012) {https://pubmed.ncbi.nlm.nih.gov/22583151/}; ; Kwon MJ, et al. Superoxide dismutase 3 suppresses hyaluronic acid fragments mediated skin inflammation by inhibition of toll-like receptor 4 signaling pathway: superoxide dismutase 3 inhibits reactive oxygen species-induced trafficking of toll-like receptor 4 to lipid rafts. (Antioxid Redox Signal. 2012) {https://pubmed.ncbi.nlm.nih.gov/21957979/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000445.1

Uniprot Accession Number

P00441

Species

Human

AA Sequence

MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ