Recombinant C.elegans SNAP25 protein
SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release. The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C. elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
23 kDa (207aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Saifee O., et al. (1998) Mol.Biol.Cell 9 (6) :1235-52.; ; Yang Y., et al. (2000) . 275 (38) :29482-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 5m MDTT, 1mM EDTA, 10%glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_505641.2
Uniprot Accession Number
O62414
Species
Human
AA Sequence
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