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Recombinant C.elegans SNAP25 protein

SNAP-25 is a presynaptic plasma membrane protein essential for synaptic vesicle fusion and neurotransmitter release. The membrane proteins SNAP-25, synaptobrevin and syntaxin make up the core complex (SNARE) essential for docking and holding synaptic vesicles at the pre synaptic membrane. SNAP-25 gene was amplified by PCR from C. elegans and cloned into an E. coli expression vector. SNAP-25 was purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Resistance to Inhibitors of Cholinesterase family member, SNAP25, SNAP-25, Synaptosomal-associated protein 25kDa, FLJ23079, RIC-4, RIC4, SEC9, SNAP 25, bA416N4.2, dJ1068F16.2, SuP, resistance to inhibitors of cholinesterase 4 homolog, super protein, synaptosomal-associated 25 kDa protein, synaptosomal-associated protein 25, RIC 4

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23 kDa (207aa) confirmed by MALDI-TOF

Additionnal Information

SNAP25, Resistance to Inhibitors of Cholinesterase family member, SNAP25, SNAP-25, Synaptosomal-associated protein 25kDa, FLJ23079, RIC-4, RIC4, SEC9, SNAP 25, bA416N4.2, dJ1068F16.2, SuP, resistance to inhibitors of cholinesterase 4 homolog, super protein, synaptosomal-associated 25 kDa protein, synaptosomal-associated protein 25, RIC 4, SNP3001-10 µg, SNP3001-20 µg, SNP3001-50 µg, SNP3001-100 µg, SNP3001-250 µg, SNP3001-500 µg, SNP3001-1 mg, SNP3001-10, SNP3001-20, SNP3001-50, SNP3001-100, SNP3001-250, SNP3001-500, SNP3001-1

References & Citations

Saifee O., et al. (1998) Mol.Biol.Cell 9 (6) :1235-52.; ; Yang Y., et al. (2000) . 275 (38) :29482-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 5m MDTT, 1mM EDTA, 10%glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_505641.2

Uniprot Accession Number

O62414

Species

Human

AA Sequence

MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERC EGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQP RITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK

Frequently Asked Questions

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