Recombinant human SNAP25 protein
The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters and this protein exists as two alternative isoforms, SNAP25A and SNAP25B which differ by 9 amino acids in central portion of these proteins. Recombinant SNAP25B protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.3 kDa (206aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Tafoya LC., et al. (2006) J. Neurosci. 26 (30) :7826-38.; ; Schulz JR., et al. (1998) J. Biol Chem. 273 (38) :24355-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_570824.1
Uniprot Accession Number
P60880
Species
Human
AA Sequence
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