Recombinant human SNAP25 protein

The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters. Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.4 kDa (226aa) confirmed by MALDI-TOF

Additionnal Information

SNAP25, Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa., SNP0601-10 µg, SNP0601-20 µg, SNP0601-50 µg, SNP0601-100 µg, SNP0601-250 µg, SNP0601-500 µg, SNP0601-1 mg, SNP0601-10, SNP0601-20, SNP0601-50, SNP0601-100, SNP0601-250, SNP0601-500, SNP0601-1

References & Citations

Tafoya LC., et al. (2006) J. Neurosci . 26 (30) :7826-38.; ; Schulz JR., . (1998) J. Biol Chem. 273 (38) :24355-9.

Other References

Kull S et al., Isolation and functional characterization of the novel Clostridium botulinum neurotoxin A8 subtype. (PLoS One. 2015) {https://pubmed.ncbi.nlm.nih.gov/25658638/}; ; von Berg L et al., Functional detection of botulinum neurotoxin serotypes A to F by monoclonal neoepitope-specific antibodies and suspension array technology. (Sci Rep. 2019) {https://pubmed.ncbi.nlm.nih.gov/30940836/}; ; Steinberg M et al., Functional detection of botulinum neurotoxin serotypes A to F by monoclonal neoepitope-specific antibodies and suspension array. (ALTEX. 2025) {https://pubmed.ncbi.nlm.nih.gov/40396553/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM HEPES buffer (pH 7.4) containing 5mM DTT, 100 mM NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_570824.1

Uniprot Accession Number

P60880

Species

Human

AA Sequence

MAEDADMRNE LEEMQRRADQ LADESLESTR RMLQLVEESK DAGIRTLVML DEQGEQLERI EEGMDQINKD MKEAEKNLTD LGKFCGLCVC PCNKLKSSDA YKKAWGNNQD GVVASQPARV VDEREQMAIS GGFIRRVTND ARENEMDENL EQVSGIIGNL RHMALDMGNE IDTQNRQIDR IMEKADSNKT RIDEANQRAT KMLGSG