Recombinant human alpha-Synuclein 112/NACP112 protein
An alternatively spliced (103-129) form of alpha-synuclein. alpha-synuclein112 was cloned into an E. coli expression vector by RT-PCR.. The recombinant protein was purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
11.3 kDa (112aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ueda K., et al. (1994) Biochem. Biophys. Res. Commun. 205 (2) :1366-72.; ; Park SM., et al. (2002) Blood. 100 (7) :2506-2514.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl
Scientific Category
Neuroscience
NCBI Accession Number
NP_000336.1
Uniprot Accession Number
P37840
Species
Human
AA Sequence
MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKEGYQDYEP EA
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