Recombinant human alpha-Synuclein (lyophilized) protein

Alpha-Synuclein (amino acids 1-140), an acidic neuronal protein of 140 amino acids, is extremely heat-resistant and is natively unfolded with an extended structure primarily composed of random coils. alpha-synuclein has been suggested to be implicated in the pathogenesis of Parkinson's disease and related neurodegenerative disorders, and more recently, to be an important regulatory component of vesicular transport in neuronal cells. Moreover, recent studies have shown that alpha-synuclein has chaperone activity and that this activity is lost upon removing its C-terminal acidic tail (amino acids 96-140) .

Product Specifications

Product Name Alternative

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

Reconstitute in sterile distilled water at a concentration of 1 mg/mL or less.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

14.4 kDa (140aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

A-Synuclein (lyophilized), SNCA, NACP, PARK1, alpha-Synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Alpha synuclein, Alpha-synuclein isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, Non-A-beta component of alzheimers disease amyloid, precursor of PARK 1, PARK 4, PARK4, Parkinson disease familial 1, PD 1, PD1, Synuclein alpha, SNA2001L-10 µg, SNA2001L-20 µg, SNA2001L-50 µg, SNA2001L-100 µg, SNA2001L-250 µg, SNA2001L-500 µg, SNA2001L-1 mg, SNA2001L-10, SNA2001L-20, SNA2001L-50, SNA2001L-100, SNA2001L-250, SNA2001L-500, SNA2001L-1

References & Citations

Jakes, R., et al. (1994) FEBS lett. 345, 27-32; ; ueda, K., et al. (1993) Proc. Natl. Acad. Sci. uSA 90, 11282-11286; ; Kim, J. (1997) Molecules and Cells 7, 78-83; ; Paik, S. R., et al. (1997) Arch. Biochem. Biophys. 344, 325-334.;

Other References

Rodriguez-Araujo G, et al. Alpha-synuclein elicits glucose uptake and utilization in adipocytes through the Gab1/PI3K/Akt transduction pathway. (Cell Mol Life Sci. 2013) {https://pubmed.ncbi.nlm.nih.gov/23124190/}; ; Kim JY, et al. Nanomolar concentration of alpha-synuclein enhances dopaminergic neuronal survival via Akt pathway. (Neural Regen Res. 2013) {https://pubmed.ncbi.nlm.nih.gov/25206648/}; ; Kim HL, et al. The presence of outer arm fucose residues on the N-glycans of tissue inhibitor of metalloproteinases-1 reduces its activity. (J Proteome Res. 2013) {https://pubmed.ncbi.nlm.nih.gov/23815085/}; ; Rodriguez-Araujo G, et al. Low alpha-synuclein levels in the blood are associated with insulin resistance. (Sci Rep. 2015) {https://pubmed.ncbi.nlm.nih.gov/26159928/}; ; Takami Y, et al. Novel pathophysiological roles of α-synuclein in age-related vascular endothelial dysfunction. (FASEB J. 2022) {https://pubmed.ncbi.nlm.nih.gov/36125010/}; ; Lu J, et al. A breakdown in microglial metabolic reprogramming causes internalization dysfunction of α-synuclein in a mouse model of Parkinsons disease. (J Neuroinflammation. 2022) {https://pubmed.ncbi.nlm.nih.gov/35599331/}; ; Wang D, et al. Nanopolyphenol rejuvenates microglial surveillance of multiple misfolded proteins through metabolic reprogramming. (Acta Pharm Sin B. 2023) {https://pubmed.ncbi.nlm.nih.gov/36873190/}; ; Lee J, et al. Targeted degradation of ⍺-synuclein aggregates in Parkinsons disease using the AUTOTAC technology. (Mol Neurodegener. 2023) {https://pubmed.ncbi.nlm.nih.gov/37355598/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Lyophilized from a 0.2 um filtered in 20 mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl, 1mM MgCl2

Scientific Category

Neuroscience

NCBI Accession Number

NP_000336

Uniprot Accession Number

P37840

Species

Human

AA Sequence

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA