Recombinant human SMAC/Diablo protein

Smac/Diablo is one of the proapoptotic proteins. Smac promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding to inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. Recombinant human Smac fused to T7-tag. at N-terminus was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Diablo isoform 1, SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein, Direct IAP binding protein with low pI, Second mitochondria derived activator of caspase.

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

SMADiablo, Diablo isoform 1, SMAC3, DIABLO-S, FLJ10537, FLJ25049, Diablo isoform 1, SMAC/Diablo, Diablo isoform 1 0610041G12Rik, DBOH, Diablo homolog, DIABLO S, Diablo homolog (Drosophila), Mitochondrial Smac protein, Diablo homolog mitochondrial, SMAC 3, Smac protein, Direct IAP binding protein with low pI, Second mitochondria derived activator of caspase., SMA0801-10 µg, SMA0801-20 µg, SMA0801-50 µg, SMA0801-100 µg, SMA0801-250 µg, SMA0801-500 µg, SMA0801-1 mg, SMA0801-10, SMA0801-20, SMA0801-50, SMA0801-100, SMA0801-250, SMA0801-500, SMA0801-1

References & Citations

Srinivasula SM., et al. (2000) . J Biol Chem. 275 (46) :36152-7.; ; Du C., et al. (2000) . Cell. 102 (1) :33-42.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Cancer

NCBI Accession Number

NP_063940

Uniprot Accession Number

Q9NR28

Species

Human

AA Sequence

MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED