Recombinant E.coli SlyD protein
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
21 kDa (196aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhang JW., et al. (2007) J Bacteriol. 189 (21) :7942-4.; ; Scholz C., et al. (2006) Biochemistry. 45 (1) :20-33.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5)
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_755987
Uniprot Accession Number
P0A9K9
Species
E.coli
AA Sequence
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