Recombinant E.coli SlyD protein

SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.

Product Specifications

Specifications

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

FKBP-type peptidyl-prolyl cis-trans isomerase, FKBP-type peptidyl-prolyl cis-trans isomerase, SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD, Histidine rich protein, PPIase, Rotamase, WHP.

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

21 kDa (196aa) confirmed by MALDI-TOF

Additionnal Information

SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase, FKBP-type peptidyl-prolyl cis-trans isomerase, SlyD, FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD, Histidine rich protein, PPIase, Rotamase, WHP., SLD0801-10 µg, SLD0801-20 µg, SLD0801-50 µg, SLD0801-100 µg, SLD0801-250 µg, SLD0801-500 µg, SLD0801-1 mg, SLD0801-10, SLD0801-20, SLD0801-50, SLD0801-100, SLD0801-250, SLD0801-500, SLD0801-1

References & Citations

Zhang JW., et al. (2007) J Bacteriol. 189 (21) :7942-4.; ; Scholz C., et al. (2006) Biochemistry. 45 (1) :20-33.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_755987

Uniprot Accession Number

P0A9K9

Species

E.coli

AA Sequence

MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH