Recombinant human Sirtulin 6/SIRT6 protein

The SIRT6 is included in class IV of the sirtuin family, homologs to the yeast Sir2 protein. The functions of human sirtuins have not yet been determined; however, in yeast sirtuins are known to regulate epigenetic gene silencing. Also SIRT6, a chromatin-associated protein, appears to be involved in DNA repair. Recombinant SIRT6, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

NAD-dependent protein deacylase sirtuin-6, Protein mono-ADP-ribosyltransferase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2-like protein 6, SIR2L6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

41 kDa (375aa) confirmed by MALDI-TOF

Additionnal Information

Mono-ADP-ribosyltransferase sirtuin-6, NAD-dependent protein deacylase sirtuin-6, Protein mono-ADP-ribosyltransferase sirtuin-6, Regulatory protein SIR2 homolog 6, SIR2 like protein 6, SIR2 related protein type 6, SIR2L6, SIR2-like protein 6, SIRT 6, SIRT6, Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae), Sirtuin 6, Sirtuin 6 Mono ADP ribosyltransferase sirtuin 6, Sirtuin type 6, Sirtuin6, SIR0901-10 µg, SIR0901-20 µg, SIR0901-50 µg, SIR0901-100 µg, SIR0901-250 µg, SIR0901-500 µg, SIR0901-1 mg, SIR0901-010, SIR0901-020, SIR0901-050, SIR0901-100, SIR0901-250, SIR0901-500, SIR0901-01M

References & Citations

Michishita E., et al. (2008) Nature. 452 (7186) :492-6.; ; Mostoslavsky R., et al. (2006) Cell. 124 (2) :315-29.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF

Scientific Category

Cell Cycle

NCBI Accession Number

NP_057623

Uniprot Accession Number

Q8N6T7

Species

Human

AA Sequence

MSVNYAAGLS PYADKGKCGL PEIFDPPEEL ERKVWELARL VWQSSSVVFH TGAGISTASG IPDFRGPHGV WTMEERGLAP KFDTTFESAR PTQTHMALVQ LERVGLLRFL VSQNVDGLHV RSGFPRDKLA ELHGNMFVEE CAKCKTQYVR DTVVGTMGLK ATGRLCTVAK ARGLRACRGE LRDTILDWED SLPDRDLALA DEASRNADLS ITLGTSLQIR PSGNLPLATK RRGGRLVIVN LQPTKHDRHA DLRIHGYVDE VMTRLMKHLG LEIPAWDGPR VLERALPPLP RPPTPKLEPK EESPTRINGS IPAGPKQEPC AQHNGSEPAS PKRERPTSPA PHRPPKRVKA KAVPS