Recombinant human SHP-1 protein

The protein coding region of the catalytic domain of SHP-1 (amino acids 243-541) was cloned into an E. coli expression vector. The catalytic domain of SHP-1 was overexpressed as insoluble protein aggregates (inclusion bodies) . The recombinant SHP-1 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid (Met) is attached at N-terminus.

Product Specifications

Specifications

Specific activity is > 8,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

Product Name Alternative

Protein tyrosine phosphatase non-receptor type 6 isoform 1, PTPN6, HCP, PTP1C, Tyrosine-protein phosphatase non-receptor type 6, EC 3.1.3.48, Protein-tyrosine phosphatase 1C, PTP-1C, Hematopoietic cell protein-tyrosine phosphatase, SH-PTP1, Protein-tyrosine phosphatase SHP-1, PTPN6, HCP, HCPH, SHP1, HPTP1C, SHP-1L EC 3.1.3.48, Protein tyrosine phosphatase non-receptor type 6 isoform 1, 70 kda SHP1L protein, Hematopoietic cell phosphatase, Hematopoietic cell protein tyrosine phosphatase, HPTP 1C, Protein tyrosine phosphatase 1C, Protein tyrosine phosphatase SHP 1, Protein tyrosine phosphatase SHP1, PTP 1C, PTPN 6, SH PTP 1, SH PTP1, SHP 1, SHP 1L, SHP1L, SHPTP 1, SHPTP1, Tyrosine protein phosphatase non receptor type 6

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 8,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

Molecular Weight

34.3 kDa (300aa) confirmed by MALDI-TOF

Additionnal Information

SHP1, SHP2001-10 µg, SHP2001-20 µg, SHP2001-50 µg, SHP2001-100 µg, SHP2001-250 µg, SHP2001-500 µg, SHP2001-1 mg, SHP2001-10, SHP2001-20, SHP2001-50, SHP2001-100, SHP2001-250, SHP2001-500, SHP2001-1

References & Citations

Shen SH., et al. (1991) Nature 352, 736-739.; ; Wu C., et al. (2003) Gene. 306,1-12.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 2mM beta-mercaptoethanol, 1mM EDTA 1mM DTT, 20%glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002822

Uniprot Accession Number

P29350

Species

Human

AA Sequence

MGFWEEFESL QKQEVKNLHQ RLEGQRPENK GKNRYKNILP FDHSRVILQG RDSNIPGSDY INANYIKNQL LGPDENAKTY IASQGCLEAT VNDFWQMAWQ ENSRVIVMTT REVEKGRNKC VPYWPEVGMQ RAYGPYSVTN CGEHDTTEYK LRTLQVSPLD NGDLIREIWH YQYLSWPDHG VPSEPGGVLS FLDQINQRQE SLPHAGPIIV HCSAGIGRTG TIIVIDMLME NISTKGLDCD IDIQKTIQMV RAQRSGMVQT EAQYKFIYVA IAQFIETTKK KLEVLQSQKG QESEYGNITY