Recombinant human SF20/MYDGF protein

SF20/25, originally identified as a product of bone marrow-derived stromal cells, was previously thought to support proliferation of lymphoid cells and was designated as interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. Recombinant human SF20/IL25 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Myeloid-derived growth factor, IL27, IL27w, Interleukin 27 working designation, C19orf10, R33729_1, IL25, Stromal cell derived growth factor, SF20, IL-25

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18 kDa (162aa) confirmed by MALDI-TOF

Additionnal Information

SF20, IL25, Stromal cell-derived growth factor, C19orf10, IL25, uPF0556, IL27, IL27w, Stromal cell-derived growth factor Chromosome 19 open reading frame 10, EuROIMAGE1875335, IL 25, IL 27, Interleukin 25, Interleukin 27 working designation, Interleukin25, R33729 1, R337291, SF20, Stromal cell derived growth factor, Stromal cell derived growth factor SF20, uncharacterized protein C19orf10 precursor., SFT0904-10 µg, SFT0904-20 µg, SFT0904-50 µg, SFT0904-100 µg, SFT0904-250 µg, SFT0904-500 µg, SFT0904-1 mg, SFT0904-10, SFT0904-20, SFT0904-50, SFT0904-100, SFT0904-250, SFT0904-500, SFT0904-1

References & Citations

Weiler T., et al. (2007) Arthritis Res Ther. 9, R30.; ; Tulin E.E., et al. (2001) J. Immunol.167 (11) :6338-47;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_061980

Uniprot Accession Number

Q969H8

Species

Human

AA Sequence

SEPTTVAFD VRPGGVVHSF SHNVGPGDKY TCMFTYASQG GTNEQWQMSL GTSEDHQHFT CTIWRPQGKS YLYFTQFKAE VRGAEIEYAM AYSKAAFERE SDVPLKTEEF EVTKTAVAHR PGAFKAELSK LVIVAKASRT EL