Recombinant human RheB protein

Ras homolog enriched in brain (RheB) is a member of the Ras superfamily that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. RheB encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RheB is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. Recombinant human RheB protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras homolog enriched in brain, RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, RheB, Ras homolog enriched in brain GTP binding protein Rheb, MGC111559, Ras homolog enriched in brain 2, RHEB 2.

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.7 kDa (197aa) confirmed by MALDI-TOF

Additionnal Information

RheB, Ras homolog enriched in brain, RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, RheB, Ras homolog enriched in brain GTP binding protein Rheb, MGC111559, Ras homolog enriched in brain 2, RHEB 2., RHB0901-10 µg, RHB0901-20 µg, RHB0901-50 µg, RHB0901-100 µg, RHB0901-250 µg, RHB0901-500 µg, RHB0901-1 mg, RHB0901-10, RHB0901-20, RHB0901-50, RHB0901-100, RHB0901-250, RHB0901-500, RHB0901-1

References & Citations

Yamagata K., et al. (1994) J Biol Chem. 269 (23) 16333-9.; ; Tabancay AP Jr., et al. (2003) . J Biol Chem. 278 (41) :39921-30.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_005605

Uniprot Accession Number

Q15382

Species

Human

AA Sequence

SMPQS KSRKIAILGY RSVGKSSLTI QFVEGQFVDS YDPTIENTFT KLITVNGQEY HLQLVDTAGQ DEYSIFPQTY SIDINGYILV YSVTSIKSFE VIKVIHGKLL DMVGKVQIPI MLVGNKKDLH MERVISYEEG KALAESWNAA FLESSAKENQ TAVDVFRRII LEAEKMDGAA SQGKSSC