Recombinant human Recoverin protein

Recoverin is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome. Recombinant human recoverin was overexpressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

RCVRN, RCV1, Recoverin, Cancer-associated retinopathy protein, Protein CAR, Recoverin, Cancer associated retinopathy protein, Cancer associated retinopathy protein antibody, CAR protein

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23 kDa (200aa) confirmed by MALDI-TOF

Additionnal Information

Recoverin, RCVRN, RCV1, Recoverin, Cancer-associated retinopathy protein, Protein CAR, Recoverin, Cancer associated retinopathy protein, Cancer associated retinopathy protein antibody, CAR protein, RCV0501-10 µg, RCV0501-20 µg, RCV0501-50 µg, RCV0501-100 µg, RCV0501-250 µg, RCV0501-500 µg, RCV0501-1 mg, RCV0501-10, RCV0501-20, RCV0501-50, RCV0501-100, RCV0501-250, RCV0501-500, RCV0501-1

References & Citations

Sampath P., et al. (2004) Cancer Control. 11, 174-80; ; Erickson MA., et al. (1998) Proc. Natl. Acad. Sci. 95, 6474-9

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 2mM MgCl2, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_002894

Uniprot Accession Number

P35243

Species

Human

AA Sequence

MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA