Recombinant human RAB5A protein

Rab5a is a member of RAS oncogene family. The small GTPase, Rab5a is a crucial regulatory component of the endocytic pathway. Activation of Rab5a is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex. Recombinant human Rab5a was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RAB5A member RAS oncogene family, Ras-related protein Rab-5A, RAS-associated protein RAB5A, RAB5

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

23 kDa (215aa) confirmed by MALDI-TOF

Additionnal Information

Rab5a, RAB0701-10 µg, RAB0701-20 µg, RAB0701-50 µg, RAB0701-100 µg, RAB0701-250 µg, RAB0701-500 µg, RAB0701-1 mg, RAB0701-10, RAB0701-20, RAB0701-50, RAB0701-100, RAB0701-250, RAB0701-500, RAB0701-1

References & Citations

Tan BK, et al. (2007) J Clin Endocrinol Metab. 92 (7) :2590-7 ; ; Yao-Borengasser A, et al. (2007) ) J Clin Endocrinol Metab. 92 (7) :2430-2. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 6.0) containing 10% glycerol, 0.1mM PMSF

Scientific Category

Cancer

NCBI Accession Number

NP_004153

Uniprot Accession Number

P20339

Species

Human

AA Sequence

MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD LTEPTQPTRN QCCSN