Recombinant human PTH protein

Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 1-84) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques

Product Specifications

Product Name Alternative

PTH, Human Parathyroid Hormone, PTH, Parathyrin, Parathormone, Parathyroid hormone, hPTH, Parathormone

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

9.55 kDa (85aa) confirmed by MALDI-TOF

Additionnal Information

PTH, PTH, Human Parathyroid Hormone, PTH, Parathyrin, Parathormone, Parathyroid hormone, hPTH, Parathormone, PTH3001-10 µg, PTH3001-20 µg, PTH3001-50 µg, PTH3001-100 µg, PTH3001-250 µg, PTH3001-500 µg, PTH3001-1 mg, PTH3001-10, PTH3001-20, PTH3001-50, PTH3001-100, PTH3001-250, PTH3001-500, PTH3001-1

References & Citations

Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88 (11), 5212-20.; ; Wingender E., et al. (1989) J. Biol. Chem. 264 (8),4367-73.;

Other References

Zhang H, et al. Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer. (Talanta. 2009) {https://pubmed.ncbi.nlm.nih.gov/19576433/}