Recombinant human Phosphoserine phosphatase/PSPH protein

Human Phosphoserine phosphatase (hPSP) is an important enzyme in the phosphorylated pathway of serine biosynthesis, which contributes a major portion of the endogenous L-serine. Similar to known L-3-phosphoserine phosphatases, it catalyzed the Mg2+ General references: -dependent hydrolysis of L-phosphoserine and an exchange reaction between L-serine and L-phosphoserine. Recently, its complex structures reveal that the open-closed environmental change of the active site, generated by local rearrangement of the alpha-helical bundle domain, is important to substrate recognition and hydrolysis. Recombinant human hPSP was overexpressed in E. coli and purified by conventional chromatography.

Product Specifications

Product Name Alternative

PSPH, Phosphoserine phosphatase Human, PSPase, PSP, EC 3.1.3.3, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, L 3 phosphoserine phosphatase, O phosphoserine phosphohydrolase, Phosphoserine phosphatase deficiency, included

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

25 kDa (225aa) confirmed by MALDI-TOF

Additionnal Information

Phosphoserine phosphatase, PSPH, PSPH, Phosphoserine phosphatase Human, PSPase, PSP, EC 3.1.3.3, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, L 3 phosphoserine phosphatase, O phosphoserine phosphohydrolase, Phosphoserine phosphatase deficiency, included, PSP0501-10 µg, PSP0501-20 µg, PSP0501-50 µg, PSP0501-100 µg, PSP0501-250 µg, PSP0501-500 µg, PSP0501-1 mg, PSP0501-10, PSP0501-20, PSP0501-50, PSP0501-100, PSP0501-250, PSP0501-500, PSP0501-1

References & Citations

Moro-Furlani., AM., et al. (1980) Ann. Hum. Genet. 43, 323-333; ; Collet JF., et al. (1997) FEBS Lett. 408, 281-284 ; ; Kim HY, et al. (2002) J Biol Chem. 277, 46651-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM HEPES buffer (pH 7.4) containing 100 mM KCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004568.2

Uniprot Accession Number

P78330

Species

Human

AA Sequence

MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE