Recombinant human PSMA7 protein

Proteasome alpha 7, PSMA7, is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMA7, belonged to the peptidase T1A family, is a 20S core alpha subunit of proteasome. This protein was found to interact specifically with two subdomains of HIF-1alpha and inhibited the transactivation function of HIF-1alpha under both normoxic and hypoxia-mimicking conditions. Recombinant PSMA7 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proteasome alpha 7 subunit, C6, HSPC, MGC3755, RC6-1, XAPC7, Proteasome alpha 7 subunit, PSMA7, Proteasome alpha 7 subunit Proteasome (prosome macropain) subunit alpha type 7, Proteasome alpha 7 subunit, Proteasome subunit alpha 4, Proteasome subunit alpha type 7, PSMA 7, RC6 1, Proteasome subunit RC6 1, Proteasome subunit XAPC7.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

30 kDa (268aa) confirmed by MALDI-TOF

Additionnal Information

PSMA7, Proteasome alpha 7 subunit, C6, HSPC, MGC3755, RC6-1, XAPC7, Proteasome alpha 7 subunit, PSMA7, Proteasome alpha 7 subunit Proteasome (prosome macropain) subunit alpha type 7, Proteasome alpha 7 subunit, Proteasome subunit alpha 4, Proteasome subunit alpha type 7, PSMA 7, RC6 1, Proteasome subunit RC6 1, Proteasome subunit XAPC7., PSM0901-10 µg, PSM0901-20 µg, PSM0901-50 µg, PSM0901-100 µg, PSM0901-250 µg, PSM0901-500 µg, PSM0901-1 mg, PSM0901-10, PSM0901-20, PSM0901-50, PSM0901-100, PSM0901-250, PSM0901-500, PSM0901-1

References & Citations

Cho S., et al. (2001) FEBS Lett. 498 (1) :62-6.; ; Dong J, et al. (2004) J Biol Chem. 279 (20) :21334-42.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002783

Uniprot Accession Number

O14818

Species

Human

AA Sequence

MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ SGGKNIELAV MRRDQSLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS