Recombinant human Peroxiredoxin 1/PRDX1 protein

PRDX1 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein is also known to be important to protect red blood cells against reactive oxygen species and in tumor prevention. Recombinant human PRDX1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Product Name Alternative

Natural killer cell-enhancing factor A, NKEF-A, Proliferation-associated gene protein, PAG, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, PAGA, PAGB, TDPX2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for minute.

Molecular Weight

24 kDa (219aa) confirmed by MALDI-TOF

Additionnal Information

Heme binding 23 kDa protein, Heme binding 23kDa protein, Macrophage 23 KDa stress protein, Macrophage 23kDa stress protein, Macrophase stress protein 22 kDa, Macrophase stress protein 22kDa, MSP 23, MSP23, Natural killer cell enhancing factor A, Natural killer cell-enhancing factor A, Natural killer enhancing factor A, NKEF A, NKEFA, NKEF-A, OSF 3, OSF3, Osteoblast specific factor 3, PAG, PAGA, PAGB, Peroxiredoxin 1, Peroxiredoxin 1 Hbp23, Peroxiredoxin1, PRDX 1, PRDX1, Proliferation associated gene A, Proliferation associated gene protein, Proliferation associated protein PAG, Proliferation-associated gene protein, PRX 1, PRX I, PRX1, PRXI, TDPX 2, TDPX2, TDX 2, TDX2, Thioredoxin dependent peroxide reductase 2, Thioredoxin dependent peroxiredoxin 1, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, TPxA Macrophase stress protein 23 kD, PRX0801-10 µg, PRX0801-20 µg, PRX0801-50 µg, PRX0801-100 µg, PRX0801-250 µg, PRX0801-500 µg, PRX08

References & Citations

Iraqui I., et al. (2008) Cancer Res. 68 (4) :1055-63.; ; Parmigiani RB., et al. (2008) Proc Natl Acad Sci u S A. 105 (28) :9633-8.; ; Kim JH., et al. (2008) Clin Cancer Res. 14 (8) :2326-33.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_002565.1

Uniprot Accession Number

Q06830

Species

Human

AA Sequence

MSSGNAKIGH PAPNFKATAV MPDGQFKDIS LSDYKGKYVV FFFYPLDFTF VCPTEIIAFS DRAEEFKKLN CQVIGASVDS HFCHLAWVNT PKKQGGLGPM NIPLVSDPKR TIAQDYGVLK ADEGISFRGL FIIDDKGILR QITVNDLPVG RSVDETLRLV QAFQFTDKHG EVCPAGWKPG SDTIKPDVQK SKEYFSKQK