Recombinant human Prolactin protein

Prolactin is a hormone synthesised and secreted by lactotrope cells in the adenohypophysis (anterior pituitary gland) . Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation) . Increased serum prolactin during pregnancy causes enlargement of the mammary glands of the breasts and increases the production of milk. Recently, prolactin has demonstrated broader roles in breast cancer development, regulation of reproductive function, and immunoregulation. Prolactin was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human Prolactin in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.5 ng/mL.

Product Name Alternative

PRL, Prolactin, Lactogenic hormone, Luteotropic hormone, Mammotropin, OTTHuMP00000017727, Prolactin precursor.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 range ≤ 0.5 ng/mL.

Molecular Weight

23 kDa (200aa) confirmed by MALDI-TOF

Additionnal Information

Prolactin, PRL, Prolactin, Lactogenic hormone, Luteotropic hormone, Mammotropin, OTTHuMP00000017727, Prolactin precursor., PRO0501-10 µg, PRO0501-20 µg, PRO0501-50 µg, PRO0501-100 µg, PRO0501-250 µg, PRO0501-500 µg, PRO0501-1 mg, PRO0501-10, PRO0501-20, PRO0501-50, PRO0501-100, PRO0501-250, PRO0501-500, PRO0501-1

References & Citations

Ben-jonathan N., et al. (1996) Endocr Rev. 17 (6) :639-69.; ; Cooke NE., et al. (1981) J. Biol. Chem. 256, 4007-4016.; ; Goffin V., et al. (1999) Mol Cell Endocrinol. 151, 79-87.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000939.1

Uniprot Accession Number

P01236

Species

Human

AA Sequence

MLPICPGGAA RCQVTLRDLF DRAVVLSHYI HNLSSEMFSE FDKRYTHGRG FITKAINSCH TSSLATPEDK EQAQQMNQKD FLSLIVSILR SWNEPLYHLV TEVRGMQEAP EAILSKAVEI EEQTKRLLEG MELIVSQVHP ETKENEIYPV WSGLPSLQMA DEESRLSAYY NLLHCLRRDS HKIDNYLKLL KCRIIHNNNC