Recombinant mouse PRMT1 protein

Protein arginine N-methyltransferase 1 (PRMT1) is a type I methyltransferase that transfers a methyl group from S-adenosylmethionine to guanidino nitrogens of arginine residues to form monomethylarginine and asymmetric dimethylarginine. Functions of type I arginine methylation in proteins may include regulation of transcription, modulation of the affinity of nucleic acid-binding proteins, regulation of interferon signaling pathways, and targeting of nuclear proteins. Mouse PRMT1 (AAF37293) shares 99. 9% sequence identity with the human proteins (NP_938074) . Recombinant mouse PRMT1, fused to His-MBP tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 30 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Product Name Alternative

Protein arginine N-methyltransferase 1 isoform 2, Histone-arginine N-methyltransferase PRMT1, Heterogeneous nuclear ribonucleoproteins methyltransferase-like 2, Hrmt1l2, Mrmt1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-MBP-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 30 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Molecular Weight

84 kDa (750aa)

Additionnal Information

R1B4, Protein arginine N-methytransferase 1, Protein arginine N-methyltransferase 1 isoform 2, Protein arginine N-methyltransferase 1 ANM 1, Protein arginine N-methyltransferase 1, Protein arginine N methyltransferase1, Protein arginine N methyltransferase 1, PRMT1 isoform2, PRMT1, PRMT 1, Mrmt1, Mrmt 1, IR1B 4, IR1 B4, Interferon receptor 1bound protein 4, Interferon receptor 1 bound protein4, Interferon receptor 1 bound protein 4, Human mRNA for suppressor for yeast mutant complete cds, Human mRNA for suppressor for yeast mutant, Hrmt1l2, HRMT1L 2, Hrmt112, HRMT1 L2, HMT2, HMT1 hnRNP methyltransferase like 2, HMT1 hnRNP methyltransferase, HMT 2, Histone-arginine N-methyltransferase PRMT1, Heterogeneous nuclear ribonucleoproteins methyltransferase-like 2, Heterogeneous nuclear ribonucleoproteins methyltransferase like2, Heterogeneous nuclear ribonucleoproteins methyltransferase like 2, HCP 1, AW214366, "S, cerevisiae like 2", "HMT1 hnRNP methyltransferase like 2 (S, cerevisiae) ", "HMT

References & Citations

Herrmann F., et al. (2005) . J Biol Chem. 280 (45) :38005-10.; ; Abramovich C., et al. (1997) . EMBO J. 16 (2) :260-6. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 40 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 4mM MgCl2, 2mM DTT, 40% glycerol

Scientific Category

Histone Related

NCBI Accession Number

NP_001239405.1

Uniprot Accession Number

Q9JIF0

Species

Mouse

AA Sequence

MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLHAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR