Recombinant human DJ-1/PARK7 protein

The DJ-1 is a ubiquitously expressed protein involved in various cellular processes including spermatogenesis and fertilization, cancer, RNA-binding, androgen-receptor signaling and oxidative stress. Mutations in the DJ-1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (Park7) . Recombinant human DJ-1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Parkinson disease protein 7, Parkinsonism associated deglycase, parkinson protein 7, Parkinson disease autosomal recessive early onset 7, Maillard deglycase, Oncogene DJ1, Protein DJ-1, DJ-1, DJ1, GATD2, Protein/nucleic acid deglycase DJ-1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24 kDa (225aa) confirmed by MALDI-TOF

Additionnal Information

DJ1, DJ-1, DJ 1, DJ1 protein, GATD2, Maillard deglycase, Oncogene DJ1, PARK7, Parkinson disease autosomal recessive early onset 7, Parkinson disease protein 7, Parkinson disease protein 7 CAP1, parkinson protein 7, Parkinsonism associated deglycase, Protein DJ-1, Protein/nucleic acid deglycase DJ-1, SP22, PARK 7, PRK0601-10 µg, PRK0601-20 µg, PRK0601-50 µg, PRK0601-100 µg, PRK0601-250 µg, PRK0601-500 µg, PRK0601-1 mg, PRK0601-010, PRK0601-020, PRK0601-050, PRK0601-100, PRK0601-250, PRK0601-500, PRK0601-01M

References & Citations

Choi J., et al. (2006) J. Biol Chem. 281 (16) :10816-24.; ; Maraganore DM., et al. (2004) Neurology. 63 (3) :550-3.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_009193.2

Uniprot Accession Number

Q99497

Species

Mouse

AA Sequence

MASK RALVILAKGA EEMETVIPVD VMRRAGIKVT VAGLAGKDPV QCSRDVVICP DASLEDAKKE GPYDVVVLPG GNLGAQNLSE SAAVKEILKE QENRKGLIAA ICAGPTALLA HEIGFGSKVT THPLAKDKMM NGGHYTYSEN RVEKDGLILT SRGPGTSFEF ALAIVEALNG KEVAAQVKAP LVLKD