Recombinant human DJ-1/PARK7 protein

PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Recombinant His tagged PPP1R14A was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18 kDa (167aa) confirmed by MALDI-TOF

Additionnal Information

Regulatory subunit 14A, Regulatory (inhibitor) subunit 14A CPI 17, Regulatory (inhibitor) subunit 14A, Protein phosphatase 1 regulatory subunit 14A, Protein phosphatase 1 regulatory (inhibitor) subunit 14A, Protein phosphatase 1, PPP1R14A, PPP1INL, PKC potentiated inhibitory protein of PP1, CPI-17, CPI17, CPI 17alpha, CPI 17 alpha, CPI-17 alpha, CPI17 alpha, PPR0801-10 µg, PPR0801-20 µg, PPR0801-50 µg, PPR0801-100 µg, PPR0801-250 µg, PPR0801-500 µg, PPR0801-1 mg, PPR0801-010, PPR0801-020, PPR0801-050, PPR0801-100, PPR0801-250, PPR0801-500, PPR0801-01M

References & Citations

Hamaguchi., et al. (2000) . Biochem. Biophys. Res Commun. 274 (3) :825-30.; ; Eto M., et al. (1997) . FEBS Lett. 410 (2-3) :356-60.; ; Lartey I., et al. (2007) . Biol Reprod. 76 (6) :971-82.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_150281

Uniprot Accession Number

Q96A00

Species

Human

AA Sequence

MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP