Recombinant human DJ-1/PARK7 protein
PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Recombinant His tagged PPP1R14A was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18 kDa (167aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 1mM DTT, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_150281
Uniprot Accession Number
Q96A00
Species
Human
AA Sequence
Frequently Asked Questions
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