Recombinant human Cyclophilin-like 1/PPIL1 protein

Peptidylprolyl isomerase (cyclophilin) -like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy. Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase-like 1, Peptidylprolyl isomerase like 1, Cyclophilin like 1, CYPL1, Rotamase PPIL1, PPIase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

19.3 kDa (174aa) confirmed by MALDI-TOF

Additionnal Information

CGI-124, Cyclophilin related gene 1, Cyclophilin-like1, CYPL1, hCyPX, MGC678, Peptidylprolyl isomerase (cyclophilin) like 1, Peptidylprolyl isomerase (cyclophilin) -like 1, Peptidylprolyl isomerase (cyclophilin) -like 1 CGI124, peptidylprolyl isomerase like 1, PPIase, PPIL1, rotamase0, PPL0901-10 µg, PPL0901-20 µg, PPL0901-50 µg, PPL0901-100 µg, PPL0901-250 µg, PPL0901-500 µg, PPL0901-1 mg, PPL0901-010, PPL0901-020, PPL0901-050, PPL0901-100, PPL0901-250, PPL0901-500, PPL0901-01M