Recombinant human Cyclophilin H/PPIH protein

Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human u4/u6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the u4/u6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 220 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Product Name Alternative

Peptidylprolyl isomerase H, Peptidylprolyl isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, SnuCyp-20, PPIase H, Rotamase H, Cyclophilin H, Peptidylprolyl isomerase H CypH, EC 5.2.1.8, MGC5016, Small nuclear ribonucleoprotein particle-specific cyclophilin H, uSACYP, usnRNP associated cyclophilin SnuCyp20

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 220 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Molecular Weight

19.2 kDa (177aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin H, PPIH, Peptidylprolyl isomerase H, Peptidylprolyl isomerase H, PPIH, CYPH, CYP20, SnuCyp-20, SnuCyp-20, PPIase H, Rotamase H, Cyclophilin H, Peptidylprolyl isomerase H CypH, EC 5.2.1.8, MGC5016, Small nuclear ribonucleoprotein particle-specific cyclophilin H, uSACYP, usnRNP associated cyclophilin SnuCyp20, PPH0901-10 µg, PPH0901-20 µg, PPH0901-50 µg, PPH0901-100 µg, PPH0901-250 µg, PPH0901-500 µg, PPH0901-1 mg, PPH0901-10, PPH0901-20, PPH0901-50, PPH0901-100, PPH0901-250, PPH0901-500, PPH0901-1

References & Citations

Reidt u., et al. (2000) J Biol Chem. 275 (11) :7439-42.; ; Horowitz DS., et al. (2002) EMBO. 21 (3) :470-80.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_006338

Uniprot Accession Number

O43447

Species

Human

AA Sequence

MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM